ZEISS LSM 780 inverted Microscope

ZEISS LSM 780 inverted Microscope

ZEISS LSM 780 inverted Microscope, GENERAL SPECIFICATIONS:

 Axio Examiner Z1 motorised upright laser scanning microscope with 7 visible laser lines
 Freely selectable ROI for laser scanning, bleaching and photoactivation.
 Scan speeds of up to 8 frames/second with 512 x 512 pixels.
 xy, xz, xyz, xyzt, lambda, line scan and spot scan modes.
 Transmitted light brightfield and incident light fluorescence using metal halide HXP light
source with filters for blue, green and red emission.
 DIC optics for 25X, 40X, and 63X objectives
 Definite Focus Unit for long-term focus stability

MICROSCOPE OBJECTIVES:

 EC Plan-Neofluar 10x/0.30 WD=5.2 mm
 LD LCI PA 25x/0.8 DIC WD=0.57 mm Imm Corr (UV)VIS-IR (Multi-Immersion (Oil,
glycerine, water))
 Objective LD C-Apochromat 40x/1.1 W Corr
 Plan Apochromat 63x/1.4 oil
 Plan Apochromat 100x/1.4 oil

LASER ILLUMINATION :

 Diode 405nm
 Argon multiline 458/488/514nm (25 mW)
 Diode 561nm
 HeNe 594 nm
 HeNe 633nm

DETECTORS:

 Descanned Detectors (Single photon) 2 x GaAsP plus 2 standard PMT NDD detectors

ENVIRONMENT CONTROL:
 Incubator chamber with temperature and CO2 control

SOFTWARE:
 ZEN Black 2011 SP7 LSM software
3D and 4D reconstruction and animation.
Analysis and quantification for co-localisation.
Software modules for FRAP, FRET, FCS, Tiles, Positions, and Regions, and Experiment Designer