ZEISS 710 Elyra confocal (inverted) Microscope
ZEISS 710 Elyra confocal (inverted) Microscope, Zeiss LSM 710 Laser scanning microscope with, 561nm,405nm, Argon multiline 640nm Laser lines. Two PMT with META
Objectives:
EC Plan-Neofluar 5x/0,16 M27,
Plan-Apochromat 10x/0,3 DIC M27
Plan-Apochromat 20x/0,8 M27
Plan-Apochromat 25x/0,8 Imm DIC M27, DIC-slider LCI PN 25x/0,8 II Plan-Apochromat 40x/1,3 Oil DIC M27, DIC-slider PA 40x/1,3 Ill Plan-Apochromat 63x/1,40 DIC PA 63x/1.40
Incubation with dark chamber
Software: Zen 2012 with Fret, Tiling and position modules. Computer, monitor Keyboard and mouse.
LSM 710 ELYRA PS.1 is a combination of an inverted laser scanning confocal microscopy 710 (LSM 710) with super resolution imaging microscopy ELYRA PS.1: photo activated localization microscopy (PALM)/Stochastic Optical Resolution Microscopy (STORM) and structured illumination microscopy (SIM). It is suitable for imaging targeting at 10-100nm resolution at single molecule level. In addition, Zen 2012 software, motorised X/Y stage, definite focus, TIRF mode make it capable of acquisition of tiling, multiple positions, time lapse, FRAP and FRET. It has a on stage environment chamber with a 35mm petri dish adaptor linked with a CO2/N2 controller for live cell imaging including hypoxic experiments. Furthermore, it comes with an offline data processing workstation.
EC Plan-Neofluar10x/0.3 M27, EC Plan-Neofluar20x/0.5 M27, Plan-Apochromat 63x/1.4 Oil DIC M27, Plan-Apochromat 100x/1.46 Oil DIC M27 Lasers: LSM 710: Diode laser 405nm (30mW), Ar/ML 458/488/514nm (35mW), HeNe 543nm (1 mW), HeNe 633 nm (5mW).SIM/PALM: HR Diode 405nm (50mW), HR Diode 488nm (100mW), HR DPSS 561nm (100mW), HR Diode 642nm (150mW) Cameras: sCMOS for SIM, EMCCD for PALM
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